AI Article Synopsis
- WRKY transcription factors are crucial for plant growth, stress responses, and defense.
- A specific WRKY gene was isolated from a wild relative of sweet potato, showing high expression in leaves and strong response to drought conditions.
- Overexpressing this gene in sweet potato significantly improved drought tolerance by increasing ABA and proline levels, enhancing antioxidant activity, and up-regulating related stress-response genes.
Article Abstract
WRKY transcription factors are one of the important families in plants, and have important roles in plant growth, abiotic stress responses, and defense regulation. In this study, we isolated a WRKY gene, , from the wild relative of sweet potato (H.B.K.) G. Don. This gene was highly expressed in leaf tissue and strongly induced by 20% PEG6000 and 100 μM abscisic acid (ABA). Subcellar localization analyses indicated that ItfWRKY70 was localized in the nucleus. Overexpression of significantly increased drought tolerance in transgenic sweet potato plants. The content of ABA and proline, and the activity of SOD and POD were significantly increased, whereas the content of malondialdehyde (MDA) and HO were decreased in transgenic plants under drought stress. Overexpression of up-regulated the genes involved in ABA biosynthesis, stress-response, ROS-scavenging system, and stomatal aperture in transgenic plants under drought stress. Taken together, these results demonstrated that plays a positive role in drought tolerance by accumulating the content of ABA, regulating stomatal aperture and activating the ROS scavenging system in sweet potato.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775875 | PMC |
| http://dx.doi.org/10.3390/ijms23020686 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!