Beyond BRCA1/2: Homologous Recombination Repair Genetic Profile in a Large Cohort of Apulian Ovarian Cancers.

Background: Pathogenic variants in homologous recombination repair (HRR) genes other than have been associated with a high risk of ovarian cancer (OC). In current clinical practice, genetic testing is generally limited to . Herein, we investigated the mutational status of both and 5 HRR genes in 69 unselected OC, evaluating the advantage of multigene panel testing in everyday clinical practice.

Methods: We analyzed 69 epithelial OC samples using an NGS custom multigene panel of the 5 HRR pathways genes, beyond the genetic screening routine of testing.

Results: Overall, 19 pathogenic variants (27.5%) were detected. The majority (21.7%) of patients displayed a deleterious mutation in , whereas 5.8% harbored a pathogenic variant in one of the HRR genes. Additionally, there were 14 (20.3%) uncertain significant variants (VUS). The assessment of germline mutational status showed that a small number of variants (five) were not detected in the corresponding blood sample. Notably, we detected one and four deleterious variants in the low-grade serous and endometrioid histology OC, respectively.

Conclusion: We demonstrate that using a multigene panel beyond improves the diagnostic yield in OC testing, and it could produce clinically relevant results.