Oligodendrocytes (OLs) are critical for myelination and are implicated in several brain disorders. Directed differentiation of human-induced OLs (iOLs) from pluripotent stem cells can be achieved by forced expression of different combinations of the transcription factors SOX10 (S), OLIG2 (O), and NKX6.2 (N). Here, we applied quantitative image analysis and single-cell transcriptomics to compare different transcription factor (TF) combinations for their efficacy towards robust OL lineage conversion. Compared with S alone, the combination of SON increases the number of iOLs and generates iOLs with a more complex morphology and higher expression levels of myelin-marker genes. RNA velocity analysis of individual cells reveals that S generates a population of oligodendrocyte-precursor cells (OPCs) that appear to be more immature than those generated by SON and to display distinct molecular properties. Our work highlights that TFs for generating iOPCs or iOLs should be chosen depending on the intended application or research question, and that SON might be beneficial to study more mature iOLs while S might be better suited to investigate iOPC biology.
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http://dx.doi.org/10.3390/cells11020241 | DOI Listing |
Metab Brain Dis
January 2025
Neurobiology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Aluminium is a common metallic toxicant that easily penetrates the brain and exerts severe pathological effects e.g., oxidative stress, inflammation and neurodegeneration.
View Article and Find Full Text PDFBiol Chem
January 2025
Cell Biology Center, Institute of Integrated Research, Institute of Science Tokyo (Formerly Tokyo Institute of Technology), S2-19, Nagatsuta 4259, Midori-ku, Yokohama, 226-8501, Japan.
The heat stress response is an essential defense mechanism in all organisms. Heat shock proteins (Hsps) are produced in response to thermal stress, with their expression levels regulated by heat shock transcription factors. In the key transcription factor σ positively regulates Hsp expression.
View Article and Find Full Text PDFInt J Gen Med
December 2024
Department of Pathology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yun Nan, People's Republic of China.
Purpose: To identify the epithelial cell centre regulatory transcription factors in the gastric cancer (GC) microenvironment and provide a new strategy for the diagnosis and treatment of GC.
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Front Immunol
December 2024
Department of Urology, The Second People's Hospital of Meishan City, Meishan, Sichuan, China.
Background: Prostate cancer (PCa) is a multifactorial and heterogeneous disease, ranking among the most prevalent malignancies in men. In 2020, there were 1,414,259 new cases of PCa worldwide, accounting for 7.3% of all malignant tumors.
View Article and Find Full Text PDFFront Pharmacol
December 2024
Institute for Cardiovascular Physiology, Goethe University Frankfurt, Frankfurt, Germany.
Introduction: Anandamide (AEA) is an endocannabinoid that has recently been recognized as a regulator of various inflammatory diseases as well as cancer. While AEA was thought to predominantly engage cannabinoid (CB) receptors, recent findings suggest that, given its protective anti-inflammatory role in pathological conditions, anandamide may engage not only CB receptors.
Methods: In this study, we studied the role of exogenous AEA in a mouse AirPouch model of acute inflammation by examining immune cell infiltrates by flow cytometry.
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