In two sequential replicates ( = 90 and = 96 feedlot finisher cattle, respectively) we measured the impact of an -based probiotic (DFM) and an altered feedlot pen environment on antimicrobial resistance among fecal enterococci in cattle fed (or, not fed) the macrolide tylosin. Diluted fecal samples were spiral-plated on plain and antibiotic-supplemented m- agar. In the first replicate, tylosin significantly ( < 0.05) increased the relative quantity of erythromycin-resistant enterococci. This effect was diminished in cattle fed the DFM in conjunction with tylosin, indicating a macrolide susceptible probiotic may help mitigate resistance. A similar observed effect was not statistically significant ( > 0.05) in the second replicate. Isolates were speciated and resistance phenotypes were obtained for and . Susceptible strains of bacteria fed as DFM may prove useful for mitigating the selective effects of antibiotic use; however, the longer-term sustainability of such an approach remains unclear.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772914PMC
http://dx.doi.org/10.3390/antibiotics11010106DOI Listing

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