Background: A collection of human-epidemiologically unrelated strains collected over a 3-year period (2016 to 2018) in Italy by the national surveillance Enter-Net Italia was analysed.
Methods: Antimicrobial susceptibility tests, including the determination of minimal inhibitory concentrations (MICs) for colistin, were performed. Colistin resistant strains were analysed by PCR to detect mobile colistin resistance () genes. In -negative serovar Enteritidis strains, chromosomal mutations potentially involved in colistin resistance were identified by a genomic approach.
Results: The prevalence of colistin-resistant strains was 7.7%, the majority (87.5%) were . Enteritidis. genes were identified only in one strain, a . Typhimurium monophasic variant, positive for both and genes in an IncHI2 ST4 plasmid. Several chromosomal mutations were identified in the colistin-resistant -negative . Enteritidis strains in proteins involved in lipopolysaccharide and outer membrane synthesis and modification (RfbN, LolB, ZraR) and in a component of a multidrug efflux pump (MdsC). These mutated proteins were defined as possible candidates for colistin resistance in -negative . Enteritidis of our collection.
Conclusions: The colistin national surveillance in spp. in humans, implemented with genomic-based surveillance, permitted to monitor colistin resistance, determining the prevalence of determinants and the study of new candidate mechanisms for colistin resistance.
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| http://dx.doi.org/10.3390/antibiotics11010102 | DOI Listing |
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