Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO-TaO layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified TaO-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an () enzyme logic gate.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8773754 | PMC |
| http://dx.doi.org/10.3390/bios12010043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Systemic evaluation of various CRISPR/Cas13 orthologs for knockdown of targeted transcripts in plants.
Genome Biol
December 2024
Hubei Hongshan Laboratory, National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China.
Article Synopsis
- The CRISPR/Cas13 system, known for its compactness and specificity in targeting RNA, shows potential for knocking down endogenous transcripts in plants, but its subtypes need more exploration.* -
- This research evaluates seven Cas13 orthologs from five subtypes, finding that RfxCas13d, Cas13x.1, and Cas13x.2 demonstrated high editing efficiencies (58-80%) and the ability to simultaneously target two RNA transcripts.* -
- The findings suggest a new method for transcriptome editing in plants, which could enhance crop improvement efforts and provide insights for functional research.*
Discovery of New 1,2,4-Triazole/1,3,4-Oxadiazole-Decorated Quinolinones as Agrochemical Alternatives for Controlling Viral Infection by Inhibiting the Viral Replication and Self-Assembly Process.
J Agric Food Chem
December 2024
State Key Laboratory of Green Pesticides, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Article Synopsis
- - Tobacco mosaic virus (TMV) is a major threat to crops globally, necessitating the search for effective antiviral agents to ensure food security.
- - Researchers developed 40 new azole-quinolinone compounds, with one showing strong anti-TMV activity, outperforming the commercial treatment ningnanmycin.
- - The effective compound inhibits TMV by reducing essential gene expression and disrupting viral particle assembly, with interactions confirmed through various scientific analyses.
Designing a recombinant coat protein to reduce tobacco mosaic virus infection in plants.
J Biomol Struct Dyn
November 2024
Department of Chemistry, Faculty of Science, Yazd University, Yazd, Iran.
The Tobacco Mosaic Virus (TMV) is a critical plant virus that can cause a significant drop in crop yield. To understand how recombinant coat-protein impacts the affinity and assembly of TMV's subunits, research is being conducted to assess the effect of recombinant protein on virus resistance. To develop a recombinant coat-protein that can lower TMV infection rates in plants, a design strategy was employed that involves creating defective viral subunits leading to incorrect assembly.
View Article and Find Full Text PDFSynthesis of Indol-3-yl Morpholino Derivatives Containing a Hydrazone Moiety as Potential Antiplant Virus Agents.
J Agric Food Chem
December 2024
State Key Laboratory of Green Pesticides, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang 550025, China.
A series of indol-3-yl morpholino derivatives containing a hydrazone moiety were designed and synthesized using indole-3-carboxylic acid as the starting material. The antiviral activities of the synthesized compounds were systematically evaluated. Compound , optimized using the comparative molecular field analysis (CoMFA) model, exhibited the most potent inhibitory activity against Tobacco mosaic virus (TMV) in vivo with a 50% effective concentration (EC) value of 69.
View Article and Find Full Text PDFA Self-Amplifying Human Papillomavirus 16 Vaccine Candidate Delivered by Tobacco Mosaic Virus-Like Particles.
ACS Appl Bio Mater
November 2024
Aiiso Yufeng Li Family Department of Chemical and Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United States.
Article Synopsis
- The study focused on a self-amplifying mRNA vaccine developed from the tobacco mosaic virus (TMV), designed to express a mutated E7 protein from human papillomavirus 16 (HPV16), which can suppress oncogenic activity.
- Immunization tests showed that this approach successfully triggered the production of E7-specific antibodies and activated both humoral and cellular immune responses, indicating TMV's capability to effectively carry mRNA vaccines while maintaining their integrity.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!