The stability of the -amylase enzyme has been improved from using the immobilization method on a bentonite matrix. Therefore, this study aims to obtain the higher stability of -amylase enzyme from ; hence, it is used repeatedly to reduce industrial costs. The procedures involved enzyme production, isolation, partial purification, immobilization, and characterization. Furthermore, the soluble enzyme was immobilized using 0.1 M phosphate buffer of pH 7.5 on a bentonite matrix, after which it was characterized with the following parameters such as optimum temperature, Michaelis constant ( ), maximum velocity ( ), thermal inactivation rate constant ( ), half-life ( ), and the change of energy due to denaturation (Δ ). The results showed that the soluble enzyme has an optimum temperature of 55°C, of 3.04 mg mL substrate, of 10.90 mole mL min, of 0.0171 min, t of 40.53 min, and Δ of 104.47 kJ mole, while the immobilized enzyme has an optimum temperature of 70°C, of 8.31 mg mL substrate, of 1.44 mole mL min, of 0.0060 min, of 115.50 min, and Δ of 107.37 kJ mole. Considering the results, the immobilized enzyme retained 42% of its residual activity after six reuse cycles. Additionally, the stability improvement of the -amylase enzyme by immobilization on a bentonite matrix, based on the increase in half-life, was three times greater than the soluble enzyme.
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http://dx.doi.org/10.1155/2022/3797629 | DOI Listing |
Comput Biol Med
January 2025
Faculty of Chemistry, University of Science (Vietnam National University, Hanoi), 19 Le Thanh Tong, Hoan Kiem, Ha Noi, Viet Nam; VNU University of Education, Vietnam National University, Hanoi, 144 Xuan Thuy, Cau Giay, Ha Noi, Viet Nam.
α-d-Glucose-conjugated thioureas 8a-w of substituted 4,6-diaryl-2-aminopyrimindines were designed, synthesized, and screened for their antidiabetic inhibitory activity. The thioureas with the strongest potential inhibitory activity included 8f (IC = 11.32 ± 0.
View Article and Find Full Text PDFPharmaceuticals (Basel)
January 2025
Department of Molecular Biology and Genetics, Faculty of Science, Kilis 7 Aralik University, 79000 Kilis, Türkiye.
: The genus is renowned for its diverse bioactive potential, yet the chemical composition and biological properties of remain inadequately explored. This study aimed to investigate the chemical profile, antioxidant capacity, and enzyme inhibitory activities of methanol extracts from various plant parts of . : Methanol extracts were obtained from leaves, stems, flowers, roots, and aerial portions of .
View Article and Find Full Text PDFMolecules
January 2025
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, China.
Lichuan black tea (LBT) is a well-known congou black tea in China, but there is relatively little research on its processing technology. Echa No. 10 is the main tea tree variety for producing LBT.
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January 2025
Department of Life, Health and Environmental Sciences, University of L'Aquila, Coppito, 67100 L'Aquila, Italy.
Arid ecosystems constitute a promising source of actinobacteria producing new bioactive molecules. This study aimed to explore different biological activities of actinomycetes isolated from the rhizosphere of L. in the Ghardaia region, Algeria.
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January 2025
State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
infection can induce necrotic enteritis and lead to significant economic loss to the chicken industry. In this study, a xylanase (Xyn10C), which effectively promotes the growth of probiotics, and a protease, which degrades the biofilm of were analyzed for their ability to alleviate -induced necrotic enteritis in broiler chickens. A total of 300 male AA chickens were divided into five treatment groups (control, no enzyme and no challenge; Cp, no enzyme, challenge; Xyn, Xyn10C plus challenge; Xyn+Am, Xyn10C+Amylase plus challenge; Xyn+Ap, Xyn10C+Alkaline protease plus challenge).
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