AI Article Synopsis
- Protein phosphatase 2A (PP2A) plays a crucial role in various physiological processes, and its catalytic subunit Ppp2ca is vital for spermatogenesis.
- By creating a conditional knockout of Ppp2ca in mice, researchers found that its deficiency disrupts spermatogonial differentiation, leading to cell death and meiotic arrest.
- Transcriptomic analysis revealed significant dysregulation of genes involved in spermatogenesis, highlighting PP2A's essential role in male fertility and contributing to our understanding of azoospermia.
Article Abstract
As a serine/threonine phosphatase, protein phosphatase 2A (PP2A) is essential in numerous physiological processes. By generating a catalytic subunit of PP2A (Ppp2ca) conditional knockout (CKO) in C57BL/6 J mice, we explored the possible mechanisms of azoospermia by focusing on meiosis initiation and spermatogenesis. The deficiency of Ppp2ca in germ cells conspicuously disturbed spermatogonial differentiation and led to pachynema arrest, accompanied by significant apoptosis in germ cells and defects in programmed double-strand break (DSB) repair. While the formation of XY body was normal, respectively. Ppp2ca-deficient spermatocytes exhibited an abnormal cohesion complex degradation of chromosome, probably contributing to cell death. Furthermore, transcriptomics analysis was conducted to prove several genes involved in spermatogenesis and exhibited transcriptional dysregulations in Ppp2ca-deficient testes. Our study demonstrates the irreplaceable role of PP2A in spermatogenesis and provides more evidences of azoospermia etiology.
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| http://dx.doi.org/10.1007/s43032-022-00843-z | DOI Listing |
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