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| http://dx.doi.org/10.1128/JB.00520-21 | DOI Listing |
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Variation of gene ratios in mock communities constructed with purified 16S rRNA during processing.
Sci Rep
December 2024
Department of Chemical Engineering, Polytechnic School, University of São Paulo, Av. Prof. Luciano Gualberto, Travessa 3, n. 380., São Paulo, SP, CEP 05508-900, Brazil.
16S ribosomal nucleic acid (16S rRNA) analysis allows to specifically target the metabolically active members of microbial communities. The stability of the ratios between target genes in the workflow, which is essential for the bioprocess-relevance of the data derived from this analysis, was investigated using synthetic mock communities constructed by mixing purified 16S rRNA from Bacillus subtilis (Bs), Staphylococcus aureus (Sa), Pseudomonas aeruginosa (Pa), Klebsiella pneumoniae (Kp) and Burkholderia cepacia (Bc) in different proportions. The RT reaction yielded one copy of cDNA per rRNA molecule for Pa, Bc and Sa but only 2/3 of the expected cDNA from 16S rRNAs of Bs and Kp.
View Article and Find Full Text PDFMachine Learning-Corrected Simplified Time-Dependent DFT for Systems With Inverted Single-t-o-Triplet Gaps of Interest for Photocatalytic Water Splitting.
J Comput Chem
January 2025
Department of Chemistry, University of Nevada Reno, Reno, Nevada, USA.
Hydrogen gas (H) can be produced via entirely solar-driven photocatalytic water splitting (PWS). A promising set of organic materials for facilitating PWS are the so-called inverted singlet-triplet, INVEST, materials. Inversion of the singlet (S) and triplet (T) energies reduces the population of triplet states, which are otherwise destructive under photocatalytic conditions.
View Article and Find Full Text PDFWhite Matter Imaging Phenotypes Mediate the Negative Causality of Mitochondrial DNA Copy Number on Sleep Apnea: A Bidirectional Mendelian Randomization Study and Mediation Analysis.
Nat Sci Sleep
December 2024
Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, People's Republic of China.
Purpose: Sleep apnea (SA), associated with absent neural output, is characterised by recurrent episodes of hypoxemia and repeated arousals during sleep, resulting in decreased sleep quality and various health complications. Mitochondrial DNA copy number (mtDNA-CN), an easily accessible biomarker in blood, reflects mitochondrial function. However, the causal relationship between mtDNA-CN and SA remains unclear.
View Article and Find Full Text PDFSurgical treatment experience of seven cases of Berry syndrome.
J Cardiothorac Surg
December 2024
Beijing Children's Hospital Capital Medical University Beijing, Beijing, China.
Objective: Berry syndrome is a group of rare congenital cardiac malformations including aortopulmonary window (APW), aortic origin of the right pulmonary artery (AORPA), interruption of the aortic arch (IAA), patent ductus arteriosus (PDA) (supplying the descending aorta) and intact ventricular septum. This paper will analyze the clinical data of 7 patients with Berry syndrome who underwent surgical treatment in our institution and discuss the one-stage surgical correction of Berry syndrome in combination with the literature.
Methods: From January 2013 to July 2024, a total of 7 children with Berry syndrome were admitted to the Cardiac Surgery Department of Beijing Children's Hospital.
Medical students' perceptions of improving physician satisfaction and patient care: a text network analysis approach.
BMC Med Educ
December 2024
Center for Chung-Ang Medical Education Resource Allocation (CAMERA), College of Medicine, Chung-Ang University, Dongjak-gu, Seoul, Republic of Korea.
Background: Physicians' job satisfaction and their relationships with patients are critical factors in modern healthcare. Understanding medical students' perceptions of these aspects is crucial for enhancing the quality of healthcare. Hence, this study examined medical students' perspectives on improving physician job satisfaction and the essential patient-oriented attitudes required for developing into skilled physicians.
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