Development and evaluation of a hydrogenated rosin (β-acryloxyl ethyl) ester-bonded silica stationary phase for high-performance liquid chromatography separation of paclitaxel from yew bark.

J Chromatogr A

Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China. Electronic address:

Published: February 2022

Paclitaxel (PTX) is a complex diterpenoid anticancer drug whose separation from yew biomass poses a significant challenge. In this study, a new stationary phase comprising hydrogenated rosin (β-acryloxyl ethyl) ester (HRE)-bonded silica (HRE@SiO) is developed to separate and purify PTX from crude yew-bark extract using high-performance liquid chromatography. In HRE@SiO, HRE molecules, which are functional ligands, are bonded to the surface of a silica gel matrix using a coupling agent, (3-mercaptopropyl)trimethoxysilane. The proposed HRE@SiO stationary phase was characterized by Fourier-transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, scanning electron microscopy, laser diffraction granulometry, and nitrogen gas adsorption. The HRE@SiO column exhibited excellent chromatographic performance, satisfactory performance reproducibility, and typical reversed-phase chromatographic behavior. An HRE@SiO column was used to separate PTX and its analogs, achieving resolutions exceeding 7.43 for consecutively eluted species. Stoichiometric displacement theory for retention (SDT-R), the van Deemter equation, and van 't Hoff plots were used to analyze the separation mechanism and properties of the HRE@SiO column. The results showed that hydrophobic interactions determine the analyte retention and the separation of PTX and its analogs on an HRE@SiO column is an exothermic process driven by enthalpy. Furthermore, an HRE@SiO column was employed to separate and purify PTX from crude yew-bark extract, increasing PTX purity from 6% to 82%. The findings of this study provide insights for developing rosin-based stationary phases for the separation of natural products.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.chroma.2022.462815DOI Listing

Publication Analysis

Top Keywords

hre@sio column
20
stationary phase
12
hydrogenated rosin
8
rosin β-acryloxyl
8
β-acryloxyl ethyl
8
high-performance liquid
8
liquid chromatography
8
hre@sio
8
separate purify
8
purify ptx
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!