Optimized protocol to detect protein UFMylation in cells and in vitro via immunoblotting.

STAR Protoc

Key Laboratory of Aging and Cancer Biology of Zhejiang Province, School of Basic Medical Sciences, Hangzhou Normal University, Hangzhou, China.

Published: March 2022

Ubiquitin-fold modifier 1 (UFM1) system is a recently identified ubiquitin-like modification with essential biological functions. Similar to ubiquitination, the covalent conjugation of UFM1 (UFMylation) to target proteins involves a three-step enzymatic cascade catalyzed sequentially by UFM1-activating enzyme 5 (UBA5, E1), UFM1-conjugating enzyme 1 (UFC1, E2), and UFM1-specific ligase 1 (UFL1, E3). Here, we provide an optimized protocol adapted to previously reported methods for detecting the UFMylation of target protein in human cells and assays respectively, with high reliability and reproducibility. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020).

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753269PMC
http://dx.doi.org/10.1016/j.xpro.2021.101074DOI Listing

Publication Analysis

Top Keywords

optimized protocol
8
ufmylation target
8
protocol detect
4
detect protein
4
protein ufmylation
4
ufmylation cells
4
cells in vitro
4
in vitro immunoblotting
4
immunoblotting ubiquitin-fold
4
ubiquitin-fold modifier
4

Similar Publications

[Elements of high-grade iatrogenic risk in care of patients with somatic symptom disorder].

Psychiatr Hung

January 2025

Rosszullétek speciális elme- és ideggyógyászati szakrendelô, Budapest, Hungary, E-mail:

Author analyses the process of care of somatic symptom disorder in the general medical practice with special concern to iatrogenic harms. In his opinion the latency of the patients' first appointment with a psychiatrist or clinical psychologist is caused by multiple factors. Patients do not have knowledge on the basic activities of central nervous system necessary to recognize the pathomechanism.

View Article and Find Full Text PDF

Molar incisor hypomineralization (MIH) presents a multifaceted challenge in pediatric dentistry, impacting diagnostics, clinical management, resource accessibility, and psychosocial care. The condition's complexity is exacerbated by diagnostic variability, overlapping clinical symptoms, and the need for tailored treatment approaches. This study aims to explore the key challenges associated with the management of MIH in pediatric dentistry, including diagnostic precision, clinical management, resource limitations, interdisciplinary care, long-term follow-up, and psychosocial impact, and to propose strategies for overcoming these obstacles.

View Article and Find Full Text PDF

Objective: This mini-review provides an overview of the current evidence for Revumenib, a first-in-class menin inhibitor, in treating AML with KMT2A rearrangements or NPM1 mutations. This therapy represents a promising advancement by selectively disrupting leukemogenic pathways.

Summary: The clinical promise of Revumenib in genetically defined AML highlights its potential role in shaping the future treatment landscape.

View Article and Find Full Text PDF

An integrative review of the effects of high-intensity interval training on the autonomic nervous system.

Sports Med Health Sci

March 2025

Laboratory of Experimental Neurosciences, University of South Santa Catarina (UNISUL), Avenida Pedra Branca n 25, 88137-900, Palhoça, Santa Catarina, Brazil.

High-Intensity Interval Training (HIIT) has gained prominence as a time-efficient and effective exercise modality to improve cardiovascular (CV) fitness, metabolic health, and physical performance. Therefore, our aim was to synthesize current clinical research on the effects of HIIT on the Autonomic Nervous System. We conducted the search for studies in the Directory of Open Access Journals, Embase, Virtual Health Library, Pubmed, and Scielo databases, in January of 2024.

View Article and Find Full Text PDF

Managing acute coronary syndrome (ACS) in patients with a recent history of gastrointestinal bleeding presents a unique and challenging clinical dilemma, necessitating a careful balance between minimizing ischemic risk and avoiding potentially life-threatening rebleeding. Standard treatment for ACS typically involves dual antiplatelet therapy (DAPT) to prevent recurrent thrombotic events. However, in patients with recent gastrointestinal hemorrhage or significant anemia, these therapies may substantially increase the risk of life-threatening bleeding, complicating the decision-making process and often leading to conservative management strategies.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!