Objective: To explore the regulation of miR-125a-5p in hepatocellular carcinoma (HCC) and its mechanisms.
Methods: By transfecting a miR-125a-5p sequence and an interfering sequence of miR-125a-5p-s into human HCC cell lines HCC-LM3 and HepG2, miR-125a-5p-related levels were assesed by Western blot. The abilities of cell proliferation and migration were assessed by cell culture and Transwell assay, respectively.
Results: HepG2 cells showed increased miR-125a-5p levels compared with HCC-LM3 cells ( < 0.01). However, compared with QZG cells, the level of miR-125a-5p in HepG2 and HCC-LM3 cells was down-regulated. Compared with miR-125a-5p groups, miR-125a-5p-s groups showed increased colony formation rate and mobility (P < 0.01). After being transfected with miR-125a-5p, the transformation factor 2β (TRA2β) and mRNA levels were decreased, whereas 5p-s expression was increased ( < 0.01). Inhibition of TRA2β by small interfering RNA (siRNA) diminished the ability of cells.
Conclusion: miR-125a-5p inhibits the invasive capacity of HCC cells through targeting the TRA2β pathway.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8748114 | PMC |
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