We develop a disposable electrochemical sensor using a titanium nanoparticles (Ti NPs)-anchored functionalized multi-walled carbon nanotube (Ti@f-MWCNTs) composite as electrochemical sensing interface for the detection of ractopamine (RAC). The sensor demonstrated superior electrochemical sensing ability with a broad linear response range (0.01-185 μM) and ultralow detection limit (0.0038 µM). In addition, the stability, repeatability, reproducibility, and anti-interference ability of the Ti@f-MWCNTs sensor were satisfactory. The practicability of the sensor was effectively employed for the determination of RAC in porcine samples including pork, pig urine, and pig serum with substantial recoveries in the range of 92%-99% and a relative standard deviation of less than 5%.
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http://dx.doi.org/10.1016/j.foodchem.2022.132083 | DOI Listing |
J Agric Food Chem
November 2024
National Key Laboratory of Veterinary Public Health and Safety, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
As the most widely used immunoassay, enzyme-linked immunosorbent assay (ELISA) relies on perishable enzymes and usually provides poor sensitivity and stability, limiting its application in detecting trace analytes in harsh environments. Herein, a new ratiometric fluorescence (RF) sensing platform enhanced by a nanozyme-enzyme cascade reaction composed of MnO nanosheets (MnO NSs) and alkaline phosphatase (ALP) was proposed. In this RF platform, the versatile MnO NSs worked as a robust oxidase-like nanozyme to catalyze nonfluorescent Amplex Red (AR) into fluorescent resorufin and as a quencher to quench the fluorescence of carbon dots (CDs).
View Article and Find Full Text PDFFood Chem
February 2025
National R & D Center for Agro-processing Equipment, College of Engineering, China Agricultural University, Beijing 100083, China.
Surface enhanced Raman spectroscopy (SERS) holds great potential due to its rapid detection and high sensitivity. However, issues such as signal noise, fluctuations, and spectral shifts can negatively impact its performance in detecting ractopamine in pork. Hierarchical Gradient Aware Spectral Network (HGASNet) was proposed to address these issues.
View Article and Find Full Text PDFFoods
October 2024
China Innovation Instrument Co., Ltd., Ningbo 315000, China.
Biosensors (Basel)
September 2024
Department of Engineering Science, National Cheng Kung University, Tainan 70101, Taiwan.
A novel microfluidic ractopamine (RAC) detection platform consisting of a microfluidic RAC chip and a smart analysis device is proposed for the determination of RAC concentration in meat samples. This technology utilizes gold nanoparticles (AuNPs) modified with glutamic acid (GLU) and polyethyleneimine (PEI) to measure RAC concentration in food products. When RAC is present, AuNPs aggregate through hydrogen bonding, causing noticeable changes in their optical properties, which are detected using a self-built UV-visible micro-spectrophotometer.
View Article and Find Full Text PDFAnal Chem
October 2024
College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China.
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