Effect of short-term frozen storage on taste of gonads of female Eriocheir sinensis and the classification of taste quality combined with sensory evaluation and fuzzy logic model.

To investigate the taste quality of gonads of Eriocheir sinensis during frozen storage, the difference in overall taste profile was determined by electronic tongue, and the contents of free amino acids and 5'-Nucleotides were measure. The results showed that the overall taste profile of samples during frozen storage could be effectively distinguished. The contents of free amino acids and 5'-Nucleotides increased with the extension of frozen storage time. The content of water-soluble substances stored at -20 ℃ was higher than those of stored at -40 and -80 ℃. In addition, taste was evaluated by equivalent umami concentrention (EUC) and taste active value (TAV). The sensory evaluation results indicated that with the extension of frozen storage time, umami, sweetness and saltiness decreased, while bitterness increased, which was inconsistent with the previous experimental results. Sensory verification experiments were carried out to prove that bitterness could inhibit umami, sweetness and saltiness. Fuzzy logic model was used to classify the taste of gonads during frozen storage, with Hypoxanthine (Hx), Lysine (Lys) and Histidine (His) as input variables and twelve flavor grades as output variables. Finally, taste was evaluated using the designed model. When the gonads were stored at -20, -40 and -80 °C, the Pearson correlation coefficients between storage time and the taste grade of gonads were 0.98, 0.99 and 0.99, respectively.