AI Article Synopsis
- A novel dual-imprinted electrochemical paper-based analytical device (Di-ePAD) has been developed to simultaneously measure biomarkers 8-OHdG and 3-NT in urine and plasma samples, enhancing detection of oxidative stress.
- The device utilizes a molecularly imprinted polymer (MIP) formed on a silica nanosphere decorated with silver nanoparticles, allowing for improved selectivity, conductivity, and catalytic activity in sensing analytes.
- The Di-ePAD demonstrates a wide detection range (0.01-500 μM for 3-NT and 0.05-500 μM for 8-OHdG) with low detection limits and shows good accuracy in real sample analysis compared to traditional methods like HPLC.
Article Abstract
We present a novel dual-imprinted electrochemical paper-based analytical device (Di-ePAD) to simultaneously determine 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 3-nitrotyrosine (3-NT) and assess oxidative and nitrative biomarkers in urine and plasma samples. The Di-ePAD was designed with hydrophobic barrier layers formed on filter paper to provide three-dimensional circular reservoirs and assembled electrodes. The molecularly imprinted polymer (MIP) was synthesized using a silica nanosphere decorated with silver nanoparticles (SiO@AgNPs) as a core covered with dual-analyte imprinted sites on the polymer to recognize selectively and bind the target biomarkers. This strategy drives monodispersity and enhances the conductivity of the resulting MIP core-shell products. 3-NT-MIP and 8-OHdG-MIP were synthesized by successively coating the surface of SiO@AgNPs with l-Cysteine via the thiol group, then terminating with MIP shells. The dual imprinted core-shell composites possess attractive properties for the target biomarkers' sensing, including catalytic activity, selectivity, and good conductivity. The Di-ePAD revealed excellent linear dynamic ranges of 0.01-500 μM for 3-NT and 0.05-500 μM for 8-OHdG, with detection limits of 0.0027 μM for 3-NT and 0.0138 μM for 8-OHdG. This newly developed method based on the synergistic effects of SiO@AgNPs combined with promising properties of MIP offers outstanding selectivity, sensitivity, reproducibility, simplicity, and low cost for quantitative analysis of 3-NT and 8-OHdG. The proposed Di-ePAD showed good accuracy and precision when applied to actual samples, including urine and serum samples validated by a conventional HPLC method.
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| http://dx.doi.org/10.1016/j.aca.2021.339363 | DOI Listing |
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