Concentrations of Cd, Hg, Pb, As, Al, Cu, Fe, and Zn were determined in the muscles of wild and farmed European seabass in Sinop markets between September and December in 2020, using inductively coupled plasma mass spectrometry after microwave digestion. In the study, iron (Fe), zinc (Zn), aluminum (Al), and copper (Cu) were found higher than the other metals both in wild and cultured Dicentrarchus labrax. These are essential elements, but excess amounts act as a poison. Arsenic (As) concentration was higher than cadmium (Cd), mercury (Hg), and lead (Pb) both in wild and cultured D. labrax. The estimated maximum total dietary intakes of these eight metals from both wild and farmed European seabass were below the maximum acceptable daily intake values set by the Turkish Food Codex and European Union Regulation. Results showed that according to metal amounts, consumption of D. labrax had no threat to consumers' health. The target hazard quotient (THQ) revealed that harmful health impacts may not occur. Furthermore, risk index (RI) indicated that there may have a lower risk of developing cancer in the future who have been exposed to Pb and As through fish intake. Although the fish are not overly contaminated, the metal level is rising. Increased amounts of heavy metals in fish in different areas could be due to an increase in farm inflow water, domestic sewage, and a number of other anthropogenic sources, all of which should be looked into further. Precautions should be made to safeguard this fish from metal contamination and to reduce the risk to human health.
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http://dx.doi.org/10.1007/s12011-021-03064-8 | DOI Listing |
Phys Chem Chem Phys
January 2025
Chemistry Division, Bhabha Atomic Research Centre, Mumbai, 400085, India.
The present study focuses on designing mutant peptides derived from the lanthanide binding tag (LBT) to enhance selectivity for trivalent actinide (An) ions over lanthanide (Ln) metal ions (M). The LBT is a short peptide consisting of only 17 amino acids, and is known for its high affinity towards Ln. LBT was modified by substituting hard-donor ligands like asparagine (ASN or N) and aspartic acid (ASP or D) with softer ligand cysteine (CYS or C) to create four mutant peptides: M-LBT (wild-type), M-N103C, M-D105C, and M-N103C-D105C.
View Article and Find Full Text PDFPhysiol Plant
January 2025
College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, China.
The gene GAD1 encodes a glutamate decarboxylase, which is a rate-limiting enzyme for the biosynthesis of endogenous γ-aminobutyrate acid (GABA), but a potential role of GAD1 in regulating cadmium (Cd) tolerance needs to be further elucidated in plants. The objective of this study was to investigate Cd tolerance of creeping bentgrass (Agrostis stolonifera) and transgenic yeast (Saccharomyces cerevisiae) or Arabidopsis thaliana overexpressing AsGAD1. The Cd-tolerant creeping bentgrass cultivar LOFTSL-93 accumulated more endogenous GABA in relation to a significant upregulation of AsGAD1 in leaf and root than the Cd-sensitive W66569 in response to Cd stress.
View Article and Find Full Text PDFAnal Chim Acta
February 2025
School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China; Department of Chemistry, Sungkyunkwan University, Suwon, 16419, Republic of Korea. Electronic address:
Background: Estriol (E3) is a common estrogen responsible for regulating the female reproductive system, but excessive amount can pose health risks to humans and wild life. Therefore, sensitive and accurate detection of estriol level is crucial. A novel competitive ECL immunosensor based on a dual signal amplification strategy of AuNPs@GO@SmMoSe and Gd(MoO) was fabricated for ultrasensitive detection of estriol.
View Article and Find Full Text PDFJ Hazard Mater
December 2024
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Cadmium (Cd) toxicity poses major challenges to rice cultivation, affecting plant growth and development. Wild rice and nanoparticles offer promising strategies to enhance Cd tolerance, yet little is known about their combined effects. This study evaluates the single segment substitution line (SG004) from Oryza glumaepatula (wild rice) and its response to Cd stress compared to cultivated rice (HJX74).
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Departamento de Micro y Nanotecnologías, Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Cto. Exterior S/N, C.U., Coyoacán, Ciudad de México C.P. 04510, Mexico.
Thermus thermophilus HB27 laccase (Tth-Lac) is a thermostable enzyme that contains a β-hairpin (Ala292-Gln307) covering the substrate entrance. We analyzed the role of this β-hairpin in the enzymatic activity of Tth-Lac through three β-hairpin mutants: two variants without the β-hairpin (C1Tth-Lac and C2Tth-Lac) and one with a partially modified β-hairpin (P1Tth-Lac). Enzymatic activity was assayed with different substrates with and without copper.
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