Toxoplasma gondii is an obligate protozoan parasite that can infect mammals and birds. Cats are the definitive host of T. gondii and have a very important role in transmission of toxoplasmosis due to the shedding of millions of unsporulated oocysts, that become infective in the environment. Since cats play a major key role in the epidemiology of toxoplasmosis, rapid and accurate diagnosis of infected cats has utmost importance. In this study, we developed a novel colorimetric loop mediated isothermal amplification (LAMP) assay detecting T. gondii RE gene and modified a previously developed colorimetric LAMP assay targeting B1 gene to detect T. gondii DNA in cat feces for the first time. The analytical sensitivity of colorimetric LAMP assays was determined using plasmid controls. The clinical sensitivities of both colorimetric LAMPs were determined using cat fecal DNA samples that were confirmed to be positive by two different real-time PCRs in our previous study. According to the results, analytical sensitivities of both assays were 1 copy plasmid/reaction. Using real-time PCR as a reference method, sensitivities of colorimetric LAMP assays targeting RE and B1 genes were 100% and 97.56% whereas specificities of both assays were 100%. Overall, the colorimetric LAMP RE assay developed in this study brings an advantage in the diagnosis of T. gondii in cat fecal samples since it has higher sensitivity, does not need for experienced personnel, and can be applied in basic laboratories or in the field.
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http://dx.doi.org/10.1016/j.cimid.2022.101745 | DOI Listing |
Sci Rep
January 2025
Department of Parasitology, Phramongkutklao College of Medicine, Bangkok, 10400, Thailand.
Meloidogyne enterolobii, a guava root-knot nematode, is a highly virulent pest in tropical and subtropical regions causing galls or knots in roots of diverse plant species posing a serious threat to agriculture. Managing this nematode is challenging due to limitations in conventional identification based on isolation and microscopic classification requiring expertise and time. A colorimetric and fluorescent LAMP assay using simplified extraction method targeting rDNA-ITS region was developed to detect M.
View Article and Find Full Text PDFJ Fungi (Basel)
December 2024
Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hunghom, Hong Kong, China.
The genus comprises fungal species closely related to , with and being medically important. These species can cause infections in both immunocompetent and immunocompromised individuals. The current detection methods are limited, prompting the need for rapid and specific diagnostic tools.
View Article and Find Full Text PDFSpectrochim Acta A Mol Biomol Spectrosc
December 2024
Department of Chemistry and Biochemistry, Thapar Institute of Engineering and Technology, Patiala 147004, India. Electronic address:
A simple, tailor-made, novel chemosensor based on 1,10-phenanthroline Schiff base incorporating N, N-Diethylamino salicylaldehyde (1) was designed and synthesized. The sensing ability of chemosensor 1 was tested via colorimetric, UV-Vis and fluorescence spectroscopy. Chemosensor 1 could effectively and specifically detect diethylchlorophosphate (DCP) in acetonitrile displaying naked eye colour change from pale yellow to dark yellow while fluorogenic colour changes from blue to pink fluorescence (365 nm UV lamp irradiation).
View Article and Find Full Text PDFBMC Bioinformatics
December 2024
Noblis, Inc., 2002 Edmund Halley Dr, Reston, VA, 20191, USA.
Background: The bacterium Vibrio cholerae causes diarrheal illness and can acquire genetic material leading to multiple drug resistance (MDR). Rapid detection of resistance-conferring mobile genetic elements helps avoid the prescription of ineffective antibiotics for specific strains. Colorimetric loop-mediated isothermal amplification (LAMP) assays provide a rapid and cost-effective means for detection at point-of-care since they do not require specialized equipment, require limited expertise to perform, and can take less than 30 min to perform in resource limited regions.
View Article and Find Full Text PDFInt J Immunopathol Pharmacol
December 2024
Department of General Surgery, The Second Hospital of Shanxi Medical University, Taiyuan, China.
To investigate the mechanism of pancreatic alveolar cell autophagy in rats with severe acute pancreatitis (SAP) by phillygenin (PHI) based on the PI3K/Akt/mToR pathway. Rats were randomly divided into control group (CON group), SAP model group (SAP group) and PHI treatment group (SAP+PHI group), with 10 rats in each group. 5% sodium taurocholate was injected retrogradely into the biliopancreatic duct to establish a SAP rat model, and PHI was injected intraperitoneally into the pancreas after successful establishment of the model.
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