AI Article Synopsis
- Detecting individual proteins optically has potential for advancing our understanding of biological processes and high-throughput applications, but traditional fluorescent labels come with limitations.
- This research explores a label-free technique using gold nanorods to monitor changes in scattered light as proteins move past them.
- The method offers high-speed observations, capturing protein interactions with a temporal resolution between nanoseconds and microseconds.
Article Abstract
Optical detection of individual proteins with high bandwidth holds great promise for understanding important biological processes on the nanoscale and for high-throughput fingerprinting applications. As fluorescent labels impose restrictions on detection bandwidth and require time-intensive and invasive processes, label-free optical techniques are highly desirable. Here, we read out changes in the resonantly scattered field of individual gold nanorods interferometrically and use photothermal spectroscopy to optimize the experiment’s parameters. This interferometric plasmonic scattering enables the observation of single proteins as they traverse plasmonic near fields of gold nanorods with unprecedented temporal resolution in the nanosecond-to-microsecond range.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759745 | PMC |
| http://dx.doi.org/10.1126/sciadv.abl5576 | DOI Listing |
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