Nosomma monstrosum Nuttall & Warburton, 1908 is a hard tick infesting mainly Asian water buffaloes, but it has also been recorded from cattle, horses, bears, dogs, wild boar and humans in the Oriental region. This tick species has previously not been recorded from Pakistan. A total of 15 adult N. monstrosum ticks (five females and ten males) were collected from an Asian water buffalo herd grazing in Haripur district, Khyber Pakhtunkhwa (KP), Pakistan. Molecular characterization using partial 12S, 16S rRNA, and cytochrome c oxidase 1 (cox1) gene sequences confirmed the identity as N. monstrosum. A phylogenetic analysis showed that N. monstrosum from Pakistan is closely related to specimens reported from Sri Lanka and Vietnam.
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