AI Article Synopsis
- Cytotoxic T lymphocytes and natural killer (NK) cells play crucial roles in fighting off intracellular infections and cancer.
- A simplified workflow for intracellular staining is introduced to help classify these cells in human blood based on their cytolytic abilities.
- Recommended reagents are provided to measure cytolytic proteins and identify various subsets of T and NK cells, enhancing our understanding of immune responses.
Article Abstract
Cytotoxic T lymphocytes and natural killer (NK) cells are key effector cells in immune defenses against intracellular pathogens and cancer. In human blood, effector T and NK cytotoxic cells comprise a diverse and relatively rare group of cells. Herein, we describe a simplified intracellular staining workflow for classification of circulating human T and NK cells with cytolytic potential. We suggest reagents for measuring cytolytic proteins and identification of cell subsets within conventional and unconventional T cells and NK cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8728532 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.101069 | DOI Listing |
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