A single layer artificial neural network type architecture with molecular engineered bacteria for reversible and irreversible computing.

Here, we adapted the basic concept of artificial neural networks (ANNs) and experimentally demonstrate a broadly applicable single layer ANN type architecture with molecular engineered bacteria to perform complex irreversible computing like multiplexing, de-multiplexing, encoding, decoding, majority functions, and reversible computing like Feynman and Fredkin gates. The encoder and majority functions and reversible computing were experimentally implemented within living cells for the first time. We created cellular devices, which worked as artificial neuro-synapses in bacteria, where input chemical signals were linearly combined and processed through a non-linear activation function to produce fluorescent protein outputs. To create such cellular devices, we established a set of rules by correlating truth tables, mathematical equations of ANNs, and cellular device design, which unlike cellular computing, does not require a circuit diagram and the equation directly correlates the design of the cellular device. To our knowledge this is the first adaptation of ANN type architecture with engineered cells. This work may have significance in establishing a new platform for cellular computing, reversible computing and in transforming living cells as ANN-enabled hardware.