Cytocompatibility of Mats Prepared from Different Electrospun Polymer Nanofibers.

ACS Appl Bio Mater

Institute for Analytical Chemistry, Chemo- and Biosensors, Faculty of Chemistry and Pharmacy, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.

Published: August 2020

AI Article Synopsis

  • Mats made from cytocompatible polymer fibers are important for tissue engineering and wound healing, and can now also be used for sensing biological conditions in tissue.
  • Electrospinning techniques allow for the creation of these fiber mats using polymers like PLA, PS, and PVP, while providing control over their structural and functional properties.
  • Cell tests showed that human keratinocytes grew well on these mats without affecting their metabolism or inducing inflammation, indicating their potential as biocompatible scaffolds for future sensing applications.

Article Abstract

Mats of cytocompatible polymer fibers are needed as scaffolds in tissue engineering or as wound healing supports. Most recently, they have emerged as matrix-material to allow for in situ chemo- and biosensing inside intact tissue fragments or surrogates. Electrospinning of such fibers from polymer solutions provides extended options to control the structural and functional properties of the resulting fiber mats. We have prepared electrospun polymeric fiber mats from poly(lactic acid) (PLA), polystyrene (PS), and poly(vinyl pyrrolidone) (PVP) with two different fiber densities. Mats and individual fibers were characterized with respect to their dimensions, morphology, and their compatibility with human keratinocytes (HaCaT) selected as a biological model. Microscopic inspection revealed that HaCaT cells were viable on mats from all three polymers with only a negligible fraction of dead cells, similar to planar control surfaces. Growth in the presence of the fiber mats did not alter cellular metabolism (ATP, redox state) and did not induce significant production of cytokines (interleukin-6 (IL-6); monocyte chemoattractant protein-1 (MCP-1)). However, we did observe that fiber density changed the overall topography of the resulting mats and led to differences in the establishment of continuous cell sheets. In conclusion, the findings support the suitability of electrospun polymeric fiber mats made from PLA, PS, or PVP as potential biocompatible matrices for future two-dimensional (2D) or three-dimensional (3D) sensing of vital parameters from tissue in health and disease.

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Source
http://dx.doi.org/10.1021/acsabm.0c00426DOI Listing

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