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Characterization of Bacterial Community Structure Dynamics in a Rat Burn Wound Model Using 16S rRNA Gene Sequencing. | LitMetric

AI Article Synopsis

  • Burns damage the skin barrier and change the bacterial community, making infections more likely; understanding these changes is critical for treatment.
  • The study utilized 16S rRNA gene sequencing to analyze bacterial community shifts in deep partial-thickness burn wounds on rats, comparing these findings to traditional bacterial culture methods.
  • Results showed a temporary decrease in bacterial richness and diversity after burns, with changes in dominant strains, and it was found that 16S rRNA sequencing provided a more accurate view of bacterial conditions in wounds than traditional cultures.

Article Abstract

Burns destroy the skin barrier and alter the resident bacterial community, thereby facilitating bacterial infection. To treat a wound infection, it is necessary to understand the changes in the wound bacterial community structure. However, traditional bacterial cultures allow the identification of only readily growing or purposely cultured bacterial species and lack the capacity to detect changes in the bacterial community. In this study, 16S rRNA gene sequencing was used to detect alterations in the bacterial community structure in deep partial-thickness burn wounds on the back of Sprague-Dawley rats. These results were then compared with those obtained from the bacterial culture. Bacterial samples were collected prior to wounding and 1, 7, 14, and 21 days after wounding. The 16S rRNA gene sequence analysis showed that the number of resident bacterial species decreased after the burn. Both resident bacterial richness and diversity, which were significantly reduced after the burn, recovered following wound healing. The dominant resident strains also changed, but the inhibition of bacterial community structure was in a nonvolatile equilibrium state, even in the early stage after healing. Furthermore, the correlation between wound and environmental bacteria increased with the occurrence of burns. Hence, the 16S rRNA gene sequence analysis reflected the bacterial condition of the wounds better than the bacterial culture. 16S rRNA sequencing in the Sprague-Dawley rat burn model can provide more information for the prevention and treatment of burn infections in clinical settings and promote further development in this field.

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Source
http://dx.doi.org/10.1093/jbcr/irab244DOI Listing

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