Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Mass spectrometric characterisation of a condensation product between porphobilinogen and indolyl-3-acryloylglycine in urine of patients with acute intermittent porphyria.
J Mass Spectrom
July 2015
Bioanalysis Research Group, IMIM, Hospital del Mar Medical Research Institute, Doctor Aiguader 88, Barcelona, 08003, Spain.
We document the presence of a previously unknown species in the urine of patients with acute intermittent porphyria (AIP). The compound was fully characterised by liquid chromatography tandem mass spectrometry. Interpretation of both full spectrum acquisition and product ion spectra acquired in positive and negative ionisation modes by quadrupole time of flight MS allowed for the identification of a condensation product arising from porphobilinogen (PBG, increased in the urine of AIP patients) and indolyl-3-acryloylglycine (IAG, derived from indolylacrylic acid and present in human urine).
View Article and Find Full Text PDFScreening for defects in tryptophan metabolism.
J Chromatogr A
February 2000
Department of Paediatrics, Laboratory of Inherited Metabolic Diseases, Medical Faculty of Charles University, Hradec Králové, Czech Republic.
We introduced a two-step procedure for the detection of defects in metabolism of tryptophan: (1) HPTLC (described previously) is suitable when starting the investigation, (2) two HPLC methods with isocratic elution and spectrophotometric detection are used at the next step, when pathological findings are to be confirmed and the individual metabolites quantified. The first method enables the assessment of tryptophan, 5-hydroxyindolylacetic acid, indolylacetic acid, indolylacryloylglycine, indolylacrylic acid and its possible precursors, namely indolyllactic and indolylpropionic acids. The second procedure is intended for the monitoring of anthranilic, 3-hydroxyanthranilic, kynurenic and xanthurenic acids, kynurenine, 3-hydroxykynurenine and indoxyl-sulfate.
View Article and Find Full Text PDFWhere does indolylacrylic acid come from?
Amino Acids
March 2000
Department of Paediatrics, Faculty of Medicine & Faculty Hospital, Charles University, Hradec Králové, Czech Republic.
In addition to the main catabolic routes of tryptophan (Trp), there exist minor and less thoroughly investigated pathways; one of these leads to indolylacrylic acid (IAcrA). IAcrA is a plant growth hormone, whereas its biological role in animals is still obscure, as is the way and site where it is formed in the organism. A two-stage production is likely: Intestinal microorganisms catabolize Trp to indole derivatives which are then absorbed and converted to IAcrA and its glycine conjugate, indolylacryloylglycine (IAcrGly).
View Article and Find Full Text PDFHigh-performance liquid chromatographic profiling of indolylacryloylglycine and its possible precursors in body fluids.
J Chromatogr A
April 1996
Department of Paediatrics, Charles University Medical Faculty Hospital, Hradec Králové, Czech Republic.
Indolylacryloylglycine (IAcrGly) is one of the physiological components of urine, although its source and its role in the human organism have not yet been unambiguously established. Changes in the IAcrGly excretion level have been found under some physiological (age dependence, seasonal variations) and pathological (photodermatoses, muscle dystrophy, liver cirrhosis) conditions. The proposed method for IAcrGly, indolylacrylic acid and its possible precursors, namely indolyllactic and indolylpropionic acids, involves deproteinization and extraction of urine on a Sep Pak C18 cartridge.
View Article and Find Full Text PDF[Metabolism of indolylacrylic acid. I. Hypaforin].
Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove Suppl
March 1988
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!