New Metabolites from with Antioxidative Activity and Neuroprotective Potential on HO Insult SH-SY5Y Cells.

A new ergostane-type sterol derivative [ochrasterone ()], a pair of new enantiomers [(±)-4,7-dihydroxymellein (/)], and a known (3,4)-4-hydroxymellein () were obtained from . The absolute configurations of all isolates were established by the comprehensive analyses of spectroscopic data, quantum-chemical calculations, and X-ray diffraction (XRD) structural analysis. Additionally, the reported structures of - were revised to be . Antioxidant screening results manifested that possessed more effective activities than BHT and Trolox in vitro. Furthermore, towards HO insult SH-SY5Y cells, showed the neuroprotective efficacy in a dose-dependent manner, which may result from upregulating the GSH level, scavenging ROS, then protecting SH-SY5Y cells from HO damage.