Community-acquired Acinteobacter baumanii (AB) infection in the pediatric population is a rare entity in developed countries. Most of the cases in literature have been reported from India and Pakistan. Few cases of pediatric AB infection have been reported in the U.S., the vast majority of which have been from the neonatal intensive care unit population. The ubiquitous nature of the organism makes it challenging to isolate and identify as a true pathogen. For this same reason, it is imperative to have a high degree of suspicion for AB infection especially in infants and immunocompromised patients particularly when no other etiologic agent is isolated. We present a case of a healthy 37-day-old full-term neonate who presented with sepsis from Acinetobacter baumanii identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. The patient responded well to timely administration of antibiotics. She received 14-day course of cefepime based on culture susceptibility with no reported complications.
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RSC Chem Biol
December 2024
School of Biomolecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology (VISTEC) Wangchan Valley Rayong Thailand
Understanding how an enzyme regulates its function through substrate or allosteric regulation is crucial for controlling metabolic pathways. Some flavin-dependent monooxygenases (FDMOs) have evolved an allosteric mechanism to produce reduced flavin while minimizing the use of NADH and the production of harmful hydrogen peroxide (HO). In this work, we investigated in-depth mechanisms of how the reductase component (C1) of -hydroxyphenylacetate (HPA) 3-hydroxylase (HPAH) from is allosterically controlled by the binding of HPA, which is a substrate of its monooxygenase counterpart (C2).
View Article and Find Full Text PDFJ Ayub Med Coll Abbottabad
November 2024
Khyber Medical College, Peshawar-Pakistan.
Surgical site infections are a common complication of surgery with a high prevalence in lower middle-income countries (LMICS). Excessive focus on antibiotic prophylaxis to prevent surgical site infections while neglecting other precautions like aseptic measures, patient nutrition, proper wound care has resulted in the emergence of antibiotic resistant microbials. Such organisms are a menace to healthcare.
View Article and Find Full Text PDFIJID Reg
December 2024
Laboratory of Microbiology, University Hospital Mohammed VI of Marrakech, Marrakech, Morocco.
J Hosp Infect
November 2024
Department of Microbiology, Virology and Infection Control, Great Ormond Street Hospital London, UK. Electronic address:
Background: Multi-resistant Gram-negative bacteria (GNB) survive in hospital drains in traps that contain water and may ascend into the sink because of splashes, or biofilm growth.
Aim: To investigate whether the 'Tuba Drain' (TD) a long, bent, continually descending copper tube between the sink outlet and the trap prevents the ascent of bacteria.
Methods: After initial laboratory tests confirmed that the TD prevented bacteria in the U-bend from splashing upwards into the sink outlet, TDs were assessed in a randomized, blinded trial in a hospital outpatient department built in 2019.
Front Immunol
October 2024
Dipartimento di Malattie Infettive, Istituto Superiore di Sanità, Roma, Italy.
The immunization of mice with the sterile culture medium supernatants of (Mtb) H37Rv permitted the production of several monoclonal antibodies (mAbs) specific for secreted and/or released antigens. Two mAbs bound and immunoprecipitated an 80-kDa protein that was identified by mass spectrometry as Rv1133c, the methionine synthase MetE. The protein MetE is ubiquitous among prokaryota and shows a significant sequence homology in many bacteria.
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