species are highly prevalent in various regions of China. The study's objective is to isolate and identify from natural habitats and compare the phylogenetic diversity of in southern Yunnan province, China. Metacercariae of was isolated from crabs, and morphologic identification was performed by microscopy. Metacercariae were injected into experimental free Sprague Dawley rats. After 114 days, adult worms and eggs were isolated from multiple organs. Morphologic identification confirmed the initial identification. DNA was extracted from 5 adult worms, and molecular characterization was performed by amplification and sequencing of CO1 and ITS2 regions, followed by phylogenetic analysis. Out of 447 crabs captured, 186 crabs were found to be infected. A total of 4 species of was observed from naturally infected crabs. (2), (1), (1), and (1) were isolated and identified. A total of 32 sequences were downloaded from the National Center for Biotechnology Information, and 5 sequences generated in the study were used for phylogenetic analysis. In the phylogenetic tree of the CO1 gene, , , and were clustered with the same species, and the confidence values of their branches were >95%. A congruent phylogenetic relationship was observed with the ITS2 phylogenetic tree. In the phylogenetic tree constructed with the combined dataset of CO1 and ITS2 datasets, , , and clustered with the same species, and their branch confidence values were >94%. clustered with in both datasets. Phylogenetic analysis revealed robustness of the double loci method as against the single-locus method with either CO1 or ITS2 alone. species isolated from the southern Yunnan province, China, was phylogenetically diverse, and the analysis revealed the clustering of multiple species of isolated from different geographic locations.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8741392 | PMC |
http://dx.doi.org/10.1155/2021/5646291 | DOI Listing |
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