Russia has started the process of correcting its GMO regulation, but it does not use the same approach to regulating genome-edited plant products as other countries. Our assessment here is based not only on science but also on important social and economic factors.
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| http://dx.doi.org/10.1016/j.tibtech.2021.12.004 | DOI Listing |
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TaDL interacts with TaB3 and TaNF-YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat.
J Integr Plant Biol
December 2024
Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Crop Heterosis and Utilization (MOE) and Beijing Key Laboratory of Crop Genetic Improvement, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193, China.
Starch biosynthesis is a critical factor in wheat (Triticum aestivum L.) quality and yield. However, the full scope of its regulation is not fully understood.
View Article and Find Full Text PDFGenetic and physiological characteristics of edited citrus and their impact on HLB tolerance.
Front Genome Ed
December 2024
Department of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, United States.
Article Synopsis
- Huanglongbing (HLB) disease, triggered by the bacterium Liberibacter asiaticus, poses a serious threat to citrus production with no existing cure, making the development of resistant cultivars essential.
- Researchers focused on the NONEXPRESSOR OF PATHOGENESIS-RELATED GENES (NPR) family, specifically modifying NPR1 and NPR3 genes in sweet orange trees to improve HLB resistance.
- The genome-edited sweet orange varieties showed enhanced vigor compared to wild-type trees under greenhouse conditions, suggesting that targeted gene editing can help in developing HLB-tolerant citrus plants, although further field tests are required to confirm these results.
CRISPR-Cas9 Mediated Gene Editing Platform Through Callus-to-Plant Regeneration and Functional Analysis of DoALA4─DoALA6 in Dendrobium officinale.
Plant Cell Environ
December 2024
College of Life Sciences, Nanjing Normal University, Nanjing, China.
Dendrobium orchids are well known for their great horticultural and medicinal values; however, the CRISPR/Cas9 gene editing system for Dendrobium species still needs to be improved. Therefore, this study aims to establish a CRISPR/Cas9-based functional validation system using Dendrobium officinale as a model species for the Dendrobium genus and to validate the DoALA4─DoALA6 genes, which may relate to growth and disease resistance. We first conducted a bioinformatics analysis of the P-type ATPase gene family in D.
View Article and Find Full Text PDFAn industrial-grade Nicotiana benthamiana line for the production of glycoproteins carrying fucose-free galactosylated N-glycans.
N Biotechnol
November 2024
Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria. Electronic address:
β1,4-galactosylation is a typical human N-glycan formation with functional impact on proteins, particularly known for IgGs. Therefore, the expression of recombinant proteins with controlled galactosylation is an important quality parameter in the biotech industry. Here we describe the establishment of a plant-based expression platform for the manufacturing of recombinant proteins carrying β1,4-galactosylated N-glycans.
View Article and Find Full Text PDFCRISPR/Cas9 ribonucleoprotein mediated DNA-free genome editing in larch.
For Res (Fayettev)
October 2024
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China.
Here, a DNA-free genetic editing approach is presented for larch by delivering ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 through particle bombardment. The detailed procedure encompasses creating a transgenic system particle bombardment for the transformation of embryogenic callus, validating the functionality of RNPs, optimizing coating and delivery techniques, enhancing somatic embryo maturation, regenerating plantlets, and precisely identifying mutants. The optimal particle bombardment parameters were determined at 1,100 psi and a distance of 9 cm and the editing efficiency of the targets was verified .
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