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Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels. | LitMetric
Chirality effects on stem cell fate were investigated in three-dimensional culture using soft fibrous hydrogels consisting of self-assembled - and -form Fmoc-Phe-Phe-Cys networks photo-cross-linked by poly(ethylene glycol) (- and -gel, respectively). Encapsulated human bone marrow-derived mesenchymal stem cells were all alive, spread, and grown in both hydrogels. Interestingly, the cells preferably spread and grew in -gel compared to in -gel under mixed induction, and cell osteogenesis and adipogenesis differentiation in -gel were suppressed compared to in -gel. These results revealed that stem cell function and fate can be regulated in three-dimensional hydrogel culture systems with chiral motifs.
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