In this paper, a DNA hydrogel with low DNA concentration, short sticky end, and good mechanical strength was simply prepared via one-pot self-assembly from two kinds of DNA building block (X- and L-shaped DNA units) chaperoned by a cationic comb-type copolymer (CCC). The gelling process was completed under physiological conditions within 1 min, and the reversible sol-gel phase transition was achieved at room temperature through the continuous addition of CCC and an anionic polymer poly(sodium vinylsulfonate). Moreover, aptamer was successfully patterned into the hydrogel system via click chemistry. Upon the addition of complementary sequences (CSs) of aptamer, the aptamer was hybridized with CSs, leading to the fast dissociation of protein from aptamer with an adjustable release rate in specific regions at prospective times. The hydrogel with excellent cytocompatibility was successfully applied to human serum, a complex matrix. The aptamer-patterned DNA hydrogel is a potential candidate for controlled protein delivery.
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Chaperone Copolymer-Assisted Aptamer-Patterned DNA Hydrogels for Triggering Spatiotemporal Release of Protein.
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October 2018
State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials and Chemical Engineering, Hainan University, Haikou 570228, PR China.
In this paper, a DNA hydrogel with low DNA concentration, short sticky end, and good mechanical strength was simply prepared via one-pot self-assembly from two kinds of DNA building block (X- and L-shaped DNA units) chaperoned by a cationic comb-type copolymer (CCC). The gelling process was completed under physiological conditions within 1 min, and the reversible sol-gel phase transition was achieved at room temperature through the continuous addition of CCC and an anionic polymer poly(sodium vinylsulfonate). Moreover, aptamer was successfully patterned into the hydrogel system via click chemistry.
View Article and Find Full Text PDFAptamer-Patterned Hydrogel Films for Spatiotemporally Programmable Capture and Release of Multiple Proteins.
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State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials and Chemical Engineering , Hainan University, Haikou 570228 , PR China.
Various hydrogels have been used for protein delivery in the treatment of human diseases. Nevertheless, it is always difficult to control the capture and release of multiple proteins in different regions and periods. This research successfully proves that multiple proteins can be captured and released from the aptamer-patterned hydrogel films with an adjustable release rate at a prospective time and in specific regions utilizing the complementary DNA strand of aptamers via photoclick chemistry and DNA hybridization.
View Article and Find Full Text PDFAn aptamer-patterned hydrogel for the controlled capture and release of proteins via biorthogonal click chemistry and DNA hybridization.
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State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, P. R. China.
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