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Paclitaxel resistance is mediated by NF-κB on mesenchymal primary breast cancer cells. | LitMetric

AI Article Synopsis

  • * The study compared two types of breast cancer cells: those with an epithelial phenotype, which showed lower resistance to paclitaxel, and those with a mesenchymal phenotype, which demonstrated significant resistance linked to specific protein markers.
  • * Findings indicated that elevated levels of NF-κB and IKK in mesenchymal cells contributed to drug resistance, and using a proteasome inhibitor (ALLN) improved paclitaxel effectiveness by inhibiting NF-κB activation, suggesting a potential strategy to enhance

Article Abstract

Paclitaxel has been used widely to treat breast cancer and other types of cancer. However, resistance is a major cause of failure for treatment and results in cancer progression. The present study investigated the association between paclitaxel resistance and the mesenchymal phenotype, using a model of primary breast cancer cells and employing four different cultures, two with an epithelial phenotype (MBCDF and MBCD17) and two with a mesenchymal phenotype (MBCDF-D5 and MBCD3). Epithelial-mesenchymal markers were evaluated by western blotting; MBCDF and MBCD17 cells expressed E-cadherin, SNAIL, Slug, and Twist, low levels of N-cadherin, but not vimentin. MBCDF-D5 and MBCD3 cells expressed N-cadherin, vimentin, and higher levels of SNAIL, and low levels of E-cadherin, Slug, and Twist. Cell viability was evaluated using a crystal violet assay after paclitaxel treatment; primary breast cancer cells with mesenchymal phenotype were resistant to paclitaxel compared with the epithelial primary breast cancer cells. Furthermore, using western blotting, it was revealed that mesenchymal cells had elevated levels of nuclear factor-κΒ (NF-κB) p65 and IκB kinase (IKK). Additionally, it was demonstrated that paclitaxel-induced degradation of the inhibitor of NF-κB, activation of NF-κB in a dose-dependent manner, and Bcl-2 and Bcl-xL upregulation. Finally, employing western blotting and crystal violet assays, the effects of the proteasome inhibitor ALLN were assessed. ALLN inhibited paclitaxel-induced NF-κB activation and restored the sensitivity to paclitaxel. Together, these data suggest that targeting the NF-κB/IKK axis might be a promising strategy to overcome paclitaxel resistance.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8721864PMC
http://dx.doi.org/10.3892/ol.2021.13168DOI Listing

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