Background: Although rescue intracytoplasmic sperm injection (r-ICSI) is extensively used worldwide, the indication of r-ICSI and its optimal timing remains obscure. This study aimed to assess the outcomes of r-ICSI following in vitro fertilization in different timings when fertilization is confirmed.
Methods: This study included 5,156 cycles (47,785 eggs). Fertilization was confirmed by polar body analysis after 4 and 6 of coincubation of the sperm and oocyte. Oocytes that underwent IVF were divided into two groups based on the time when a second polar body was detected in more than 30% of all oocytes (Four- group and six- group). If the second polar body was not detected or was present in less than 30% of all oocytes after six of coincubation, rescue-ICSI (r-ICSI) was performed for oocytes without a second polar body (r-ICSI group).
Results: The fertilization rates of two pronuclear (2PN) oocytes in the three groups (Four- group, six- group, and r-ICSI group) were 70.7%, 51.3%, and 58.0%, respectively. The blastocyst formation rates were 62.8%, 53.4%, and 42.9%, respectively.
Conclusion: Performing r-ICSI after six of coincubation can salvage cases with fertilization failure in IVF. The higher fertilization rate of r-ICSI indicates that all oocytes without signs of fertilization after six of coincubation should undergo r-ICSI.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669413 | PMC |
| http://dx.doi.org/10.18502/jri.v22i4.7650 | DOI Listing |
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