Engineering a Synthetic Pathway for Tyrosol Synthesis in .

Tyrosol is an aromatic compound with great value that is widely used in the food and pharmaceutical industry. In this study, we reported a synthetic pathway for converting -coumaric acid (-CA) into tyrosol in . We found that the enzyme cascade comprising ferulic acid decarboxylase (FDC1) from , styrene monooxygenase (SMO), styrene oxide isomerase (SOI) from , and phenylacetaldehyde reductase (PAR) from could efficiently synthesize tyrosol from -CA with a conversion rate over 90%. To further expand the range of substrates, we also introduced tyrosine ammonia-lyase (TAL) from to connect the synthetic pathway with the endogenous l-tyrosine metabolism. We found that tyrosol could be efficiently produced from glycerol, reaching 545.51 mg/L tyrosol in a tyrosine-overproducing strain under shake flasks. In summary, we have established alternative routes for tyrosol synthesis from -CA (a potential lignin-derived biomass), glucose, and glycerol.