Lateral flow immunoassay (LFIA) is the most effective real-time detection method for aflatoxin B (AFB). Here, we constructed a nanozyme-strip based on MnO nanosheets (MnO NSs) as a catalytic label for detection of AFB. By taking advantage of the MnO-TMB catalytic amplified system, the new test achieves rapid detection with high sensitivity and ultrawide range. The limit of detection of the assay was 15 pg mL, which was over 100-fold lower than the maximum limit set by the European Union (EU) of AFB in foods. In addition, the strip test could offer 7 dynamic detection ranges, spanning 4 orders of magnitude, which could cater to the varieties of limits on AFB residues in foods and feeds set by different countries. The estimated recoveries were in the range of 85.67%-106.38% with coefficients of variations (CVs) less than 9.68%. Overall, the developed approach is a rapid, reliable, sensitive and widely available tool for on-site detection of AFB.
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