AI Article Synopsis
- The article discusses two widely used clinical methods, ELISA for protein detection and qPCR for nucleic acid analysis, highlighting their limitations like low sensitivity and complex procedures.
- It introduces DNA nanotechnology as a promising alternative that offers high accuracy and simplicity for cancer biomarker detection.
- The review summarizes various bioassay strategies leveraging DNA nanotechnology and explores their potential for future clinical applications.
Article Abstract
The most commonly used clinical methods are enzyme-linked immunosorbent assay (ELISA) and quantitative PCR (qPCR) in which ELISA was applied for the detection of protein biomarkers and qPCR was especially applied for nucleic acid biomarker analysis. Although these constructed methods have been applied in wide range, they also showed some inherent shortcomings such as low sensitivity, large sample volume and complex operations. At present, many methods have been successfully constructed on the basis of DNA nanotechnology with the merits of high accuracy, rapid and simple operation for cancer biomarkers assay. In this review, we summarized the bioassay strategies based on DNA nanotechnology from the perspective of the analytical attributes for the first time and discussed and the feasibility of the reported strategies for clinical application in the future.
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| http://dx.doi.org/10.1016/j.semcancer.2021.12.012 | DOI Listing |
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