AI Article Synopsis
- Studies show that DNA-protein interactions play crucial roles in regulating processes like replication, repair, and transcription, affecting gene expression and potentially leading to tumors.
- Chromatin immunoprecipitation assay (ChIP) is the primary method used to study DNA-binding in living organisms and has become essential in cancer research.
- The article focuses on using ChIP with a pediatric medulloblastoma cell line (DAOY) to analyze transcription factors, histone modifications, and discover new therapeutic targets.
Article Abstract
Studies of DNA-protein interactions have revealed regulatory mechanisms of DNA replication, repair, remodeling, and transcription. Perturbation of any or all of these processes result in differential gene expression that can lead to tumor development. Chromatin immunoprecipitation assay (ChIP), currently the only method available to explore DNA-binding in vivo, has become a vastly utilized tool for cancer research. In this article we discuss an assay specified for a pediatric medulloblastoma (MB) cell line DAOY used to determine binding of transcription factors, to detect histone modifications, and to identify novel therapeutic targets.
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| http://dx.doi.org/10.1007/978-1-0716-1952-0_4 | DOI Listing |
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