Active or Autoclaved Relieves TNF-α-Induced Inflammation in Intestinal Epithelial Cells Through Distinct Pathways.

Intestinal inflammation is a major threat to the health and growth of young animals such as piglets. As a next-generation probiotics, limited studies have shown that could alleviate inflammation of intestinal epithelial cells (IECs). In this study, a TNF-α-induced inflammatory model of IPEC-J2 cells, the intestinal porcine enterocytes, was built to evaluate the effects of active or inactive on the inflammation of IECs. The viability of IPEC-J2 cells was the highest when treated with active (10 copies/mL) or inactive (10 copies/mL) for 7.5 h ( < 0.01). Treated with 20 ng/mL of TNF-α and followed by a treatment of , the mRNA level of proinflammatory cytokines (, and ) was remarkably reduced ( < 0.05) along with the increased mRNA level of tight junction proteins ( and , < 0.05). Flow cytometry analysis showed that active or inactive significantly suppressed the rate of the early and total apoptotic of the inflammatory IPEC-J2 cells ( < 0.05). According to results of transcriptome sequencing, active and inactive may decline cell apoptosis by down-regulating the expression of key genes in calcium signaling pathway, or up-regulating the expression of key genes in cell cycle signaling pathway. And the bacterium may alleviate the inflammation of IECs by down-regulating the expression of PI3K upstream receptor genes. Our results indicate that may be a promising NGP targeting intestinal inflammation.