AI Article Synopsis

  • - Timely start of antimicrobial therapy is crucial for patients with bloodstream infections to decrease death and illness rates, and the study aims to evaluate errors in a specific antimicrobial susceptibility testing (AST) method.
  • - Conducted over 6 months in a large hospital in South India, the study analyzed 120 bacterial isolates from blood cultures using the automated Vitek-2 compact system against a reference method from bacterial colonies.
  • - The results showed high agreement rates (97% for Enterobacteriaceae and 97.6% for nonfermenters) between the two testing methods, suggesting that direct AST from blood culture broth is a simpler option that can speed up results by a full day, improving patient care.

Article Abstract

Timely initiation of antimicrobial therapy in patients with blood stream infection is absolutely necessary to reduce mortality and morbidity. Most clinical microbiology laboratories use conventional methods for identification and antimicrobial susceptibility testing (AST) that involve biochemical methods for identification followed by AST by disk diffusion. The aim of the current study is to assess the various errors associated with direct susceptibility testing done from blood culture broth using automated AST system-Vitek-2 compact compared with the reference method of AST done from bacterial colonies. The study was conducted in a tertiary care public sector 2,200-bedded hospital in South India for a period of 6 months. The study involved positively flagged blood culture bottles that yielded single morphotype of Gram-negative organism by Gram stain. A total of 120 bacterial isolates were collected that consisted of consecutively obtained first 60 isolates of Enterobacteriaceae family (30 and 30 ) and consecutively obtained first 60 nonfermenters (30 and 30 Vitek-2 AST was done from these 120 blood culture broth, following the protocol by Biomerieux, and results were obtained. Then, Vitek-2 was done from colonies (reference method) using appropriate panel for Enterobacteriaceae and nonfermenters, and results were obtained. Both the results were compared. Nonfermenters showed a better categorical agreement of 97.6%, as compared to Enterobacteriaceae, which showed 97%. Among Enterobacteriaceae, both and showed categorical agreement of 97% each. The procedure of AST directly from blood culture broth represents a simple and effective technique that can reduce the turnaround time by 24 hours, which in turn benefits the clinician in appropriate utilization of antimicrobials for better patient care.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8714412PMC
http://dx.doi.org/10.1055/s-0041-1732489DOI Listing

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