Effects of orexin A on PTGS2, PTGES, CBR1 and PGFS mRNA transcript abundances and prostaglandin E and F concentrations in culture medium of pig uterine explants collected during early gestation and the estrogenic cycle.

In this study, aims were to evaluate orexin A (OXA) effects on mRNA abundance of important enzymes involved in prostaglandin production, such as cyclooxygenase 2 (PTGS2), microsomal PGE synthase-1 (PTGES), PGF synthase (PGFS) and carbonyl reductase 1 (CBR1), as well as prostaglandin E (PGE) and F (PGF) culture medium concentrations for endometrial and myometrial explants. Tissues were collected from gilts during specific phases of the estrogenic cycle or early gestational period. There were greater concentrations of PGE with OXA treatments of endometrial tissues collected on days 12-13 and 27-28, as well as PGF on days 10-11 of the gestational period. The PGF concentrations were less in tissues collected on days 27-28 of the gestational period. The OXA treatments resulted in lesser concentrations of PGE from myometrial tissues collected on days 10-11 and greater PGF on days 10-11 of the gestational period and 10-11 of the estrogenic cycle. Effects of OXA may occur due to actions at PTGS2, PTGES, PGFS and CBR1 genes because mRNA abundances for proteins encoded by these genes were affected by OXA. Results indicate there is an OXA effect on mRNA abundances and prostaglandin culture medium concentrations of uterine tissue collected at different stages of the gestational period or estrogenic cycle using different doses of OXA. It, therefore, is concluded OXA may affect de novo synthesis and secretion of PGE and PGF in the uterus of pigs.