AI Article Synopsis
- - A polysaccharide called ALP4-2, rich in arabinan and derived from Atractylodes lancea, has a molecular weight of 5.59 × 10^5 g/mol and a unique structure made up of various sugars linked in complex ways.
- - Its structure includes different glycosidic linkages and differs from typical pectin due to its high methyl ester content and branched arabinan components.
- - ALP4-2 is shown to enhance immune responses by increasing phagocytosis in macrophages and stimulating the release of important cytokines and nitric oxide without causing cytotoxic effects.
Article Abstract
An arabinan-rich acidic polysaccharide, named ALP4-2 ([α]20 D = +197.8 (c 1.0 mg/mL, HO); and M = 5.59 × 10 g/mol), was obtained from Atractylodes lancea (Thunb.) DC. ALP4-2 is mainly comprised of Ara along with a small amount of GalA, Gal, Rha, Glc and Xyl. The structure was decorated by glycosidic linkages of α-Araf-(1→, →3)-α-Araf-(1→, →5)-α-Araf-(1→, →3,5)-α-Araf-(1→, →2,4)-α-Rhap-(1→, α-GalAp-(1→, →4)-α-GalAp-6-OMe-(1→, →4)-α-GalAp-6-OMe and β-Galp-(1→ with a ratio of 6:1:7:5:5:1:7:1:4. The structure, configuration and microstructure of ALP4-2 was proposed by comprehensive considerations of results from SEC-MALLS-RID, SEC-HRMS, GC-MS, and 1D/2D NMR spectroscopy. Except for a high methyl ester in full pectin regions, an abundant arabinan moiety is observed in ALP4-2 with highly complex and branched characteristics. The immunoactivity displayed that ALP4-2 can significantly promote phagocytosis of macrophage without cytotoxicity, and stimulate nitric oxide and cytokines (TNF-α, IL-6 and IL-10) release on RAW 264.7.
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| http://dx.doi.org/10.1016/j.ijbiomac.2021.12.109 | DOI Listing |
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