AI Article Synopsis
- The study analyzed how nicotine, cotinine, and anabasine affect steroid production in rat adrenal glands using various experimental techniques.
- The results showed that nicotine and anabasine led to specific changes in enzyme activity related to steroid synthesis, while cotinine had different effects.
- The findings indicate that substances found in cigarette smoke can disrupt normal adrenal hormone production, which could have implications for health.
Article Abstract
The effects of nicotine, cotinine and anabasine on rat adrenal steroidogenesis were examined by spectral and enzymatic techniques. The addition of nicotine, cotinine or anabasine to preparations of rat adrenal mitochondria produced type II cytochrome P-450 difference spectra. The addition of nicotine or anabasine, but not cotinine, to rat adrenal microsomes yielded type II cytochrome P-450 difference spectra. Nicotine and anabasine competitively inhibited rat adrenal mitochondrial 11 beta-hydroxylase and microsomal 21-hydroxylase. Cotinine competitively inhibited mitochondrial 11 beta-hydroxylase, but did not inhibit microsomal 21-hydroxylase. The apparent enzymatic inhibition constants for cotinine, nicotine, anabasine and metyrapone inhibition of the mitochondrial 11 beta-hydroxylase were 32, 96, 120 and 74 microM respectively. These studies suggest that components of cigarette smoke may alter patterns of adrenal steroidogenesis.
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| http://dx.doi.org/10.1016/0022-4731(87)90119-1 | DOI Listing |
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