The emergence of multidrug-resistant microorganisms has been termed one of the most common global health threats, emphasizing the discovery of new antibacterial agents. To address this issue, we engineered peptides harboring "RWWWR" as a central motif plus arginine (R) end-tagging and then tested them and . Our results demonstrate that Pep 6, one of the engineered peptides, shows great potential in combating bacteremia and the skin burn infection model, which induces a 62-90% reduction in bacterial burden. Remarkably, after long serial passages of and for 30 days, Pep 6 is still highly efficient in killing pathogens, compared with 64- and 128-fold increase in minimal inhibitory concentrations (MICs) for vancomycin and polymyxin B, respectively. We also found that Pep 6 exhibited robust biofilm-inhibiting activity and eliminated 61.33% of the mature methicillin-resistant (MRSA) biofilm with concentration in the MIC level. These results suggest that the RWWWR motif and binding of arginine end-tagging could be harnessed as a new agent for combating multidrug-resistant bacteria.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsami.1c19305DOI Listing

Publication Analysis

Top Keywords

arginine end-tagging
12
multidrug-resistant bacteria
8
engineered peptides
8
novel arginine
4
end-tagging antimicrobial
4
antimicrobial peptides
4
peptides combat
4
combat multidrug-resistant
4
bacteria emergence
4
emergence multidrug-resistant
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!