Proteins with clusters of CH zinc finger domains (CH-proteins) constitute the most abundant class of transcription factors in higher eukaryotes. N-terminal ZAD (zinc finger-associated domain) dimerization domain has been identified in a large group of CH-proteins mostly in insects. The piragua gene encodes one of these proteins, Fu2. We have generated CRISPR/Cas9-mediated deletion of the piragua gene that has no phenotype. We have used φC31-mediated attP/attB recombination to generate a transgenic line expressing Fu2 protein fused with HA epitope. This line will be useful for analysis of DNA binding profile and functions of Fu2 protein.
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Institute of Gene Biology, Russian Academy of Sciences, 119334, Moscow, Russia.
Proteins with clusters of CH zinc finger domains (CH-proteins) constitute the most abundant class of transcription factors in higher eukaryotes. N-terminal ZAD (zinc finger-associated domain) dimerization domain has been identified in a large group of CH-proteins mostly in insects. The piragua gene encodes one of these proteins, Fu2.
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View Article and Find Full Text PDFpiragua encodes a zinc finger protein required for development in Drosophila.
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Departamento de Neurobiología del Desarrollo y Neurofisiología, Instituto de Neurobiología, UNAM, Campus UNAM Juriquilla, Boulevard Juriquilla 3001, Querétaro, Querétaro c.p. 76230, Mexico. Electronic address:
We isolated and characterized embryonic lethal mutations in piragua (prg). The prg locus encodes a protein with an amino terminus Zinc Finger-Associated-Domain (ZAD) and nine CH zinc fingers (ZF). prg mRNA and protein expression during embryogenesis is dynamic with widespread maternal contribution, and subsequent expression in epithelial precursors.
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