Antibiotic resistance is a major challenge worldwide and increased resistance to quinolones in is being reported. Analysis of antibiotic resistance was performed on 157 strains (123 and 34 ) from conventional and organic chickens produced in Sweden. Susceptibility for tetracycline, ciprofloxacin, erythromycin, nalidixic acid, streptomycin, and gentamycin was determined by microdilution. All 77 isolates from organic chickens were sensitive to all antibiotics, except two that were resistant to tetracycline. Of the 80 isolates from conventional chickens, 22.5% of and 11.1% of were resistant to quinolones and 5.6% of were resistant to tetracycline. Whole-genome sequencing resulted in 50 different sequence types of and six of Nine sequence types were found in both organic and conventional chickens. Two of these (ST-19 and ST-257) included isolates from conventional broilers with different resistance phenotypes to the remaining isolates from conventional and organic broilers. There are management differences between the production systems, such as feed, breed, use of coccidiostats, and access to outdoor area. It is unlikely that quinolone resistance has arisen due to use of antimicrobials, since fluoroquinolones are not permitted in Swedish broiler production.
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http://dx.doi.org/10.3390/pathogens10121630 | DOI Listing |
Anal Chem
January 2025
Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong 999077, China.
Sample pretreatment for mass spectrometry (MS)-based metabolomics and lipidomics is normally conducted independently with two sample aliquots and separate matrix cleanup procedures, making the two-step process sample-intensive and time-consuming. Herein, we introduce a high-throughput pretreatment workflow for integrated nontargeted metabolomics and lipidomics leveraging the enhanced matrix removal (EMR)-lipid microelution 96-well plates. The EMR-lipid technique was innovatively employed to effectively separate and isolate non-lipid small metabolites and lipids in sequence using significantly reduced sample amounts and organic solvents.
View Article and Find Full Text PDFJ Phys Chem Lett
January 2025
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, United States.
Dark-field and confocal approaches to circular dichroism (CD) spectroscopy of uniaxial thin films examine the relationship between symmetry and incoherence in the nonreciprocal CD response, or the component that is antisymmetric about the light propagation direction. Modifying a conventional CD spectrometer for low-angle scattering detection isolates incoherent contributions to nonreciprocal CD of drop-cast thin films, boasting 5-to-10-fold enhancements in CD dissymmetry parameters. Conversely, confocal detection suppresses the nonreciprocal CD response.
View Article and Find Full Text PDFVet Q
December 2025
Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Pullorum, enabling precise differentiation from other serovars.
View Article and Find Full Text PDFInfect Prev Pract
March 2025
Central Department of Biotechnology, Tribhuvan University, Kirtipur, Nepal.
Introduction: Meticillin resistant (MRSA) is a major contributor to surgical site infections in post-operative patients. Hospital environments harbor MRSA, contributing to higher risk of nosocomial infections. Meticillin resistance is conferred by acquisition of gene, typically carried on mobile genetic element called Staphylococcal Cassette Chromosome (SCC).
View Article and Find Full Text PDFFront Microbiol
January 2025
Laboratory of Molecular Biotechnology, National Center for Biotechnology, Astana, Kazakhstan.
fungal species are considered major plant pathogens, infecting various crops and resulting in significant agricultural losses. Additionally, these species can contaminate grain with multiple mycotoxins that are harmful to humans and animals. Efficient pest management relies on timely detection and identification of phytopathogens in plant and grain samples, facilitating prompt selection of a crop protection strategy.
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