AI Article Synopsis
- Pyometra (P) can lead to serious health issues like sepsis and multiple organ dysfunction, which are identified through the role of Toll-like receptors (TLRs) in recognizing pathogens.
- A study was conducted on 200 female mice to evaluate the effects of TLR-7 and -9 via the MYD88 pathway and the NFκB response before and after ovariohysterectomy (RP), along with any lung injuries.
- Results showed significant changes in blood chemistry and immune responses, indicating that the immune system's reaction is tied to the TLR9-MYD88 pathway, marking a notable discovery in the localization and expression of TLRs and their role in lung injury related to pyometra.
Article Abstract
Background: Pyometra (P) leads to sepsis and multiple organ dysfunction syndrome. Toll-like receptors (TLRs) recognize pathogens which can cause P. The aim of this study was to investigate TLR-7 and -9 via the MYD88 pathway and the nuclear factor kappa B (NFκB) response in the uterus of a P mouse model before and after ovariohysterectomy (RP) as well as potential lung injury.
Materials And Methods: 200 female C57BL/6J mice were randomly divided into groups (N = 10/subgroup; sham 1, 2, 3, 7; P1, 2, 3, 7; 1RP1, 2, 3, 7; 2RP1, 2, 3, 7; 3RP1, 2, 3, 7) according to the day of euthanasia. Pathogens were administrated in the groups P and RP in order to induce P.
Results: Alterations in blood chemistry, histopathology, and RT-qPCT analysis before (P) and after RP were observed. Significant correlations were also found between MYD88, NFκB, and TLR9 in P and RP groups in the lungs and in RP groups in the uterus, suggesting that the immune system responded via the TLR9-MYD88 pathway.
Conclusions: This is the first report of immunohistochemical TLR-7 and -9 localization and of TLR-7, -9, MYD88, and NFκB mRNA expression in the uterus causing lung injury in a P mouse model.
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| http://dx.doi.org/10.1159/000519425 | DOI Listing |
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