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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats. | LitMetric

AI Article Synopsis

  • Hydrocarbon pollutants are harmful to the environment and difficult to break down, but certain bacteria have the ability to metabolize these substances.
  • Scientists study aquatic ecosystems to find bacteria that can help degrade these pollutants, utilizing their diverse metabolic pathways and enzymes for larger-scale applications.
  • The article provides a detailed protocol for isolating, identifying, and testing bacterial species from aquatic environments to assess their ability to use hydrocarbons as a carbon source, aimed at aiding researchers in biotechnological efforts.

Article Abstract

Hydrocarbon pollutants are recalcitrant to degradation and their accumulation in the environment is toxic to all life forms. Bacteria encode numerous catalytic enzymes and are naturally capable of metabolizing hydrocarbons. Scientists harness biodiversity in aquatic ecosystems to isolate bacteria with biodegradation and bioremediation potential. Such isolates from the environment provide a rich set of metabolic pathways and enzymes, which can be further utilized to scale up the degradation process at an industrial scale. In this article, we outline the general process of isolation, propagation, and identification of bacterial species from aquatic habitats and screen their ability to utilize hydrocarbons as the sole carbon source in vitro using simple techniques. The present protocol describes the isolation of various bacterial species and their subsequent identification using the 16S rRNA analysis. The protocol also presents steps for characterizing the hydrocarbon degrading potential of bacterial isolates. This protocol will be useful for researchers trying to isolate bacterial species from environmental habitats for their biotechnological applications.

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Source
http://dx.doi.org/10.3791/63101DOI Listing

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