We have developed a protocol for barcoded cDNA libraries of 48 samples to study gene expression across tissues in the domestic dog, by modifying the Single-Cell Tagged Reverse Transcription (STRT) protocol (Islam et al., 2012, 2014). The cDNA reads represent mRNA 5' ends, enabling the study of transcription start sites (TSS). Our modifications include longer UMIs for molecular counting and Globin-Lock® to deplete globin mRNAs that are abundant in blood and blood-rich tissues dominating all reads.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672047 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2021.100995 | DOI Listing |
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