7-Hydroxy questin, a new anthraquinone from a wetland fungus PJ03-11.

One new anthraquinone, questinlin (), four known anthraquinones (), together with three known benzophenone derivatives () were isolated from a wetland fungus PJ03-11. Their structures were elucidated on the basis of detailed spectroscopic analysis, including 1D, 2D NMR (HMQC, HMBC), mass spectrometry, and optical rotation and IR spectra. In addition, the cytotoxic activities against three human cancer cell lines (HepG2, Caco-2 and A549) were evaluated. In this article, PJ03-11, a wetland soil fungus isolated from Honghaitan, Panjin city, Liaoning province, was used to study the secondary metabolites through one strain many compounds (OSMAC) methods. By changing the culture medium and adding the substance stimulating the metabolism of the strain into the fermentation medium, the peak type and amount of ethyl acetate extract in the fermentation medium were studied by HPLC. Finally, 2 M MgCl was added into the rice solid medium for the expansion fermentation.