AI Article Synopsis
- Type 1 diabetes (T1D) leads to symptoms like increased hunger and high blood sugar levels, along with a stressed hormonal response.
- Researchers tested whether injecting a gene therapy vector for leptin (a hormone that helps regulate hunger and energy) could improve these issues in diabetic rats.
- Results showed that leptin gene therapy reduced blood sugar and some neuroendocrine dysfunctions in diabetic rats, but higher doses are needed for it to be a practical treatment for T1D.
Article Abstract
Type 1 diabetes (T1D) is characterized by hyperphagia, hyperglycemia and activation of the hypothalamic-pituitary-adrenal (HPA) axis. We have reported previously that daily leptin injections help to alleviate these symptoms. Therefore, we hypothesized that leptin gene therapy could help to normalize the neuroendocrine dysfunction seen in T1D. Adult male Sprague Dawley rats were injected i.v. with a lentiviral vector containing the leptin gene or green fluorescent protein. Ten days later, they were injected with the vehicle or streptozotocin (STZ). HPA function was assessed by measuring norepinephrine (NE) levels in the paraventricular nucleus (PVN) and serum corticosterone (CS). Treatment with the leptin lentiviral vector (Lepvv) increased leptin and insulin levels in non-diabetic rats, but not in diabetic animals. There was a significant reduction in blood glucose levels in diabetic rats due to Lepvv treatment. Both NE levels in the PVN and serum CS were reduced in diabetic rats treated with Lepvv. Results from this study provide evidence that leptin gene therapy in STZ-induced diabetic rats was able to partially normalize some of the neuroendocrine abnormalities, but studies with higher doses of the Lepvv are needed to develop this into a viable option for treating T1D.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8703968 | PMC |
| http://dx.doi.org/10.3390/ijms222413197 | DOI Listing |
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