The biosynthetic gene cluster (BGC) from the well-studied organism was studied by heterologous expression in the fungal host . Coexpression of and produces two new acyl tetramic acids. Addition of the ring-expanding cytochrome P450 encoded by then yields a known pyridone intermediate to ilicicolin H and a new chain-truncated shunt metabolite. Finally, addition of the intramolecular Diels-Alderase encoded by affords a mixture of 8-epi ilicicolin H and ilicicolin H itself, showing that the BGC encodes biosynthesis of this potent antifungal agent. Unexpected shunt pathways are responsible for the production of the new compounds, emphasising the role of fungal hosts in catalysing diversification reactions.
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| http://dx.doi.org/10.3390/jof7121034 | DOI Listing |
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